Neurochemical Characterisation of Lamina II Inhibitory Interneurons that Express GFP in the PrP-GFP Mouse
نویسندگان
چکیده
منابع مشابه
Neurochemical characterisation of lamina II inhibitory interneurons that express GFP in the PrP-GFP mouse
BACKGROUND Inhibitory interneurons in the superficial dorsal horn play important roles in modulating sensory transmission, and these roles are thought to be performed by distinct functional populations. We have identified 4 non-overlapping classes among the inhibitory interneurons in the rat, defined by the presence of galanin, neuropeptide Y, neuronal nitric oxide synthase (nNOS) and parvalbum...
متن کاملGABAergic inhibitory interneurons in the posterior piriform cortex of the GAD67-GFP mouse.
Gamma-aminobutyric acid (GABA)-releasing inhibitory interneurons, a critical component of cortical circuitry, are involved in myriad known functional roles. However, information regarding the cytoarchitectural, physiological, and molecular properties of interneurons in posterior piriform cortex (PPC) is sparse. Taking advantage of the glutamic acid decarboxylase (GAD)67-enhanced green fluoresce...
متن کاملMolecular and Electrophysiological Characterization of GFP-Expressing CA1 Interneurons in GAD65-GFP Mice
The use of transgenic mice in which subtypes of neurons are labeled with a fluorescent protein has greatly facilitated modern neuroscience research. GAD65-GFP mice, which have GABAergic interneurons labeled with GFP, are widely used in many research laboratories, although the properties of the labeled cells have not been studied in detail. Here we investigate these cells in the hippocampal area...
متن کاملPreferential origin and layer destination of GAD65-GFP cortical interneurons.
To identify the origin and track the migratory pathway of specific subpopulations of GABAergic interneurons, we studied tangential migration in a recently developed GAD65-GFP transgenic mouse strain. First, we used immunohistochemical methods to characterize the expression of specific neurochemical markers in the GAD65-GFP neurons. Then, organotypic cultures were used in combination with birth-...
متن کاملIdentification and Characterisation of a pH-stable GFP
Green fluorescent proteins (GFPs) are invaluable tools for modern cell biology. Even though many properties of GFP have been successfully engineered, a GFP retaining brightness at low pH has not emerged. This limits the use of GFP in quantitative studies performed in fluctuating or acidic conditions. We report the engineering and characterisation of tandem dimer GFP (pH-tdGFP), a bright and sta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Pain
سال: 2013
ISSN: 1744-8069,1744-8069
DOI: 10.1186/1744-8069-9-56